Phospho explorer antibody array

WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). WebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three …

Adhesion GPCR Latrophilin-2 Specifies Cardiac Lineage ... - PubMed

WebAug 3, 2024 · A phospho-protein array analysis revealed that PF-3758309 down-regulates the NF-κB signaling pathway, which provides the most likely mechanism by which PF-3758309 inhibits latency reversal. WebMar 25, 2016 · Phospho-protein profiling by Phospho Explorer Antibody Array Cell lysates obtained from PC-1 and PC-1.0 cells were applied to a Phospho Explorer Antibody Array (Full Moon Biosystems, Sunnyvale, CA, USA). The array contained 1318 antibodies. trying something new gif https://ryan-cleveland.com

Phosphokinase Antibody Arrays on Dendron-Coated Surface

WebNov 14, 2024 · The Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. WebMoon BioSystems’ antibody arrays allow researchers to detect and analyze hundreds of proteins simultaneously on a single slide, saving precious resources and reducing the number of variables that affect experimental outcome. Our unique collection of antibody arrays includes phospho-specific arrays WebJun 4, 2024 · Phosphoprotein array analysis was performed using the Phospho Explorer Antibody Array (cat. no. PEX100; Full Moon BioSystems, Inc.). Signaling protein analysis was performed using the Signaling Explorer Antibody Array (cat. no. SET100; Full Moon BioSystems, Inc.) and the Explorer Antibody Array (cat. no. ASB600; Full Moon … phil levine pensions and investments

Cell Signalling - how is the Phospho Explorer antibody array used ...

Category:Frontiers Phospho-Protein Arrays as Effective Tools for …

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Phospho explorer antibody array

Proteome Profiler™ Antibody Arrays: R&D Systems

WebProteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research. This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single … WebPhospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and … Antibody Array Assay Kit is designed for easy and reliable processing of antibody … Chen P, Huang H, Bone marrow stromal cells protect acute myeloid leukemia … Signaling Explorer Antibody Array features 1358 unique antibodies covering 20 cell … Signaling Explorer Array: Garnett D, Caveolae as a target to quench …

Phospho explorer antibody array

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Web2 Citations. Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS. $901.00. Size. In stock. SKU. AAH-PPP-1-2.

WebMar 18, 2024 · The Phospho Explorer Antibody Array kit used in this study was obtained from Full Moon BioSystems (catalog PEX100; Sunnyvale, CA, USA) and covered 1318 well-characterized antibodies, the phosphorylated and non-phosphorylated state of these proteins will be detected at the same time (Pulito et al. 2016 ). WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ...

WebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. Learn more about cytokine … WebSep 20, 2024 · The anti-ERK1/2 antibody was designed against the epitopes with the same phospho-sites as those detected by the phospho-array. All FFPE samples showed strong immunoreactivity for ERK1/2, with diffuse or regional positivity, and these results were in accordance with the very high relative phosphorylation of ERK2 as detected by the …

WebPhospho Explorer Array is a glass-based Array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples. It is a high-throughput ELISA based antibody microarray for qualitative/semi-quantitative protein …

Webarray antibody pairs are optimised to provide the most accurate and reliable data. Human Cytokine Array Panel A (Cat# ARY005B) PBMCs untreated (top) or treated with ... p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP ... trying soundtrackWebWe used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. We identified CDK5, Src, and P38MAPK as key downstream … phil levis phrmaWebCompared to the conventional methods, our antibody arrays provide a much more cost-effective means for protein research. Investigators can choose from over 40 different arrays to quickly compare protein expression or … tryings only good withWebApr 29, 2024 · Phosphoprotein profiling by the Phospho Explorer Antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA), contains 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along with multiple positive and negative controls. phil levin attorneyWebApoptosis Phospho Antibody Array This array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study serine/threonine and tyrosine phosphorylation at specific sites. phil levy mdWeb北京智杰方远科技主要为科研领域提供分子生物学、细胞生物学、抗体等相关产品。公司主要代理:Invitrognen、SIGMA、Roche、Abcam、QIAGEN等品牌。我们以“团结诚信,求实创新,质量为本,服务第一”为经营理念。在生命科学领域不断探索,希望不断发展的智杰方远能成为 … trying stuff until it worksWebTo understand the role of phosphoproteomic signaling in carvedilol's anticancer activity, the mouse epidermal JB6 P+ cells treated with EGF, carvedilol, or their combination were analyzed using the Phospho Explorer Antibody Array containing 1318 site-specific and phospho-specific antibodies of over 30 signaling pathways. trying soundtrack season 3