Dylight 550 spectra
WebSpectrum [DyLight 405] DyLight 405 is a fluorescent compound with an excitation peak at 399 nm and an emission peak at 434 nm. It can be excited using a 405 nm laser paired with a 450/50 nm bandpass filter, a configuration that can be found, for example, in the BD Special Order LSRFortessa™ Cell Analyzer. DyLight 405 is spectrally similar to ... WebForm / Storage: Conjugate supplied as a liquid in 0.1M sodium phosphate, 0.15M NaCl (pH7.4), 1% BSA, 0.02% NaN3. Upon receipt, store at 2-8°C in the dark.
Dylight 550 spectra
Did you know?
WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … WebStorage: Upon receipt store DyLight NHS Esters at -20°C. Products are shipped with an ice pack. Store all dyes in the foil pouch with desiccant to protect from light and moisture. …
WebProtein A DyLight™680 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight ... WebIHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550.
WebJan 10, 2024 · TRITC is excited with light in the green spectrum with a maximum at 550 nm. Its emission maximum is lying at 573 nm. The bond to proteins (e.g. antibodies) is also based on a reactive isothiocyanate group. ... DyLight 405: 399 nm: 434 nm: DyLight 350: 332 nm: 435 nm: BFP (Blue Fluorescent Protein) 380 nm: 439 nm: Alexa 350: 343 nm: … WebJan 21, 2010 · Absorption and fluorescence emission spectra of DyLight 594 free and labeled on IgG are presented in Fig. 1.This dye can be conveniently excited from 530 to 610 nm.Many excitation sources like a green 532 nm, 543 nm and 568 nm lasers, as well as Rhodamine 6G dye laser operate in this spectral region.Most types of photodetectors are …
WebFAQs for Lightning-Link (R) Rapid DyLight 550 Kit (323-0005). (Showing 1 - 8 of 8 FAQ). Can I label a peptide/protein/antibody other than IgG? Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would …
WebJan 1, 2007 · The excitation and emission spectra of the DyLight dyes are compatible with common laser and filter sets, and these dyes have similar performance to other commercially available fluorophores ... ravensworth rdWebThe DyLight® fluorophore spectra are shown below. Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum … ravensworth road mottinghamWeb19775 Belmont Executive Plz. 19775 Belmont Executive Plz Ashburn VA 20147-4197. Share. More. Directions. Advertisement. See a problem? simple alto sax sheet musicWebThe excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into ... (488 nm) laser for DyLight 488, a green (526 nm) laser for … simple altered book ideasWebCF® Dye Spectrum: CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. ... 555, ATTO 550, Cy®3, DyLight® 549, Rhodamine. Features. Brighter than Cy®3 Highly water-soluble Validated in multicolor STORM super-resolution imaging. simple alternatives bankWebType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. ravensworth property for saleWebThe Thermo Scientific™ DyLight™ PEGylated Dyes display absorption maxima spectra ranging from 550nm to 783nm (Table 1). These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor ... DyLight 550, CF555 : DyLight 650-4xPEG . 658 / 681 . 250,000 . 1425 : Alexa Fluor 647, Cy5, DyLight 650, CF647 . DyLight 680-4xPEG . … ravensworth road doncaster