Cyto-gcamp6f

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August undefined, 2024

WebCyto-GCaMP6f expression when driven by Aldh1l1- Cre/ERT2 in Ai95 mice at ~P66 was dependent on the tamoxifen dose administered in vivo. A. Representative images showing cyto-GCaMP6f expression as a function of tamoxifen dose in hippocampal CA1, V1 visual cortex and striatal brain sections. B. WebDec 3, 2024 · Consistent with the high efficiency and specificity of GCaMP6f labeling in our previous studies (Qin et al., 2024; Zhang et al., 2024), the microinjection of this virus resulted in reliable and...

Excitatory neurotransmission activates compartmentalized calcium ...

WebDec 16, 2024 · To verify loss of calcium transients in neurons and Müller glia following BAPTA-AM application, we include imaging results from PDGFRα;cyto-GCaMP6f flox mice (PDGFRα-Cre: strain 013148), which label Müller glia and a subset of neurons with cytosolic GCaMP6f, as well as results from Slc1a3-CreER;Lck-GCaMP6f flox retinas (Figure … WebIn addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression … city center ice skating san ramon

Addgene: pGP-CMV-GCaMP6f

WebThe main difference between Lck-GCaMP6f and cyto-GCaMP6f with respect to the startle-evoked calcium signals was in the areas that the calcium signals encompassed: the areas were significantly ... WebBased on immunohistochemical analysis, GCaMP6f expression was almost colocalized with the astrocyte marker s100β (Figures 1C,E) and not detected within neurons (Figures 1D,F) in either the cortex... WebSep 21, 2024 · (A and B) Cre −/− and Cre +/− macrophages were transduced Cyto-GCaMP6f (A) or Mito-GCaMP6f (B) and then incubated with CellVue Claret-labeled ACs. Confocal microscopy was used to capture time-lapse images every 5 min, with measurements beginning when an AC fell into the plane of focus of a macrophage in the … city center hyderabad

Excitatory neurotransmission activates compartmentalized …

Expression of AAV5-GfaABC 1 D-cyto-GCaMP6f-SV40

WebMar 17, 2024 · Gq-induced Ca 2+ is necessary for sleep-wake transitions. ( A) Experimental setup. Mice were co-injected with GFAP-cyto-GCaMP6f and GFAP-hM3D (Gq)-mCherry AAVs. After I.P. injection of either 1 mg/kg CNO or saline, 2P astrocyte Ca 2+ dynamics, LFP, EMG, and … see more Figure 6—figure supplement 1 A B see more Figure … WebJan 7, 2024 · product information Catalog Number : 52925 Product Name : pZac2.1 gfaABC1D-cyto-GCaMP6f article : bacterial resistance : Ampicillin cloning : growth strain : Fluorescent reporter for calcium signaling origin : 37 pi : resistance markers : 753 tags : High Copy more info or order : Addgene product webpage company information Addgene dick vitale bracket predictionsWebNov 10, 2024 · In this study, we expressed highly sensitive calcium indicators, GCaMP6f [ 9] and R-CaMP2 [ 10] in astrocytes and neurons, respectively, to observe simultaneously the astrocytic and neuronal visual responses in the V1 of awake mice. We found that neuromodulator-mediated global activities dominated in the astrocytic activities. city center iguazu s. a

"WebSep 30, 2024 · Two 300 nl injections (600 nl total virus) of AAV5-GFaABC1D.cyto-GCaMP6f were made into visual cortex (0.5–1.0 mm anterior and 1.75–2.5 mm lateral of bregma) at a depth of 0.2–0.3 mm and 0.5 ... " - Cyto-gcamp6f

Cyto-gcamp6f

Brain-state dependent astrocytic Ca2+ signals are coupled to both ...

WebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions … WebNov 10, 2024 · Astrocytes show visual responses at their somata. (A) Average images of cyto-GCaMP6f-expressing astrocytes. Regions colored in blue indicate the individual …

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WebJul 11, 2024 · pZac2.1 gfaABC1D-cyto-GCaMP6f in Mouse (AAV5) Addgene #52925-AAV5. Caption:Olympus Confocal microscopy, 488nm laser, XLPLN25XWMP2. Data … WebJul 29, 2024 · pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5) Promoter GFAP Injected Titer 3.60E+13 GC/ml Injected Volume 30 nl/site, 8 sites per animal, 240 nl total Injection Rate …

WebAAV-gfaABC1D-cyto-GCaMP6f $ 350.00. AAV serotypes: Clear: AAV-gfaABC1D-cyto-GCaMP6f quantity. Add to cart. SKU: construct-1250 Categories: AAV, Virus. Description Additional information Reviews (0) Description. 1 unit = 100 µl. Backorder products can be produced in approximately 6 weeks time. ... WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck-GCaMP6f flox retinas at P10 following incubation in vehicle or 200 µM BAPTA-AM. Lck-GCaMP6f fluorescence is localized to Müller glial processes.

WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck-GCaMP6f flox retinas at P10 following incubation in vehicle or 200 µM BAPTA-AM. Lck-GCaMP6f fluorescence is localized to Müller glial processes. WebDec 3, 2024 · The fluorescent genetically encoded Ca 2+ indicator (GCaMP6f) was excited by light (wavelength of 488 nm) delivered by a laser (OBIS 488 LX-50 mW, Coherent, Santa Clara, CA, USA), and the beam intensity of the laser was accurately adjusted by the current.

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WebGCaMP6f was expressed by viral injection into the forepaw somatosensory cortex (FP-S1) of the rat brain, specifically into neurons or astrocytes (Fig. 1A). The sensory-evoked Ca … city center i married an angelWebLck-GCaMP6f revealed entire bushy astrocytes, whereas cyto-GCaMP6f revealed the somata, major branches and branchlets (Supp Figure 3 A,B; n = 4 mice). Moreover, with live slice imaging we could... city center huntsvilleWebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions and fine processes ( e.g. , astrocyte processes). After Cre exposure, bright GCaMP6f fluorescence is observed following calcium binding (such as neuronal ... city center iguazúWebJul 11, 2024 · AAV2/5-gfaABC1D-cyto-GCaMP6f (Addgene 52925); viral titer 6.13 × 10 13. 5. Stereotaxic apparatus . 6. Microinjector. 7. Needle/glass micropipettes. 8. Isoflurane. 9. Scalpels. 10. Surgical drill. 11. Heat blanket. 12. Mouse body temperature probe. 2.2 Optical Fiber Implantations 1. dick vitale cancer foundationWebJan 14, 2024 · We used viral vectors expressing GCaMP6f under the control of the regulatory element from the GFAP gene (AAV5/pZac2.1 gfaABC1D-cyto-GCaMP6f, prepared by Addgene, a gift from Dr. Baljit Khakh ... city center in charlotte ncWebDec 21, 2016 · Cyto-GCaMP6f and Lck-GCaMP6f expression was sparse with Slc1a3-Cre/ERT mice, but expression of both GECIs was ubiquitous and high with Aldh1l1 … city center in anchorage alaskaWebSep 1, 2024 · A craniotomy was made to expose the visual cortex, and AAV2/5-gfaABC1D-cyto-GCaMP6f was injected to transduce cortical astrocytes for visualization of astrocytic Ca 2+ signals. Two-photon imaging of basal astrocyte Ca 2+ activity was conducted through a cranial window, 3 to 4 weeks later. city center ice cream newport news