WebApr 23, 2024 · A chalcone isomerase-like protein enhances flavonoid production and flower pigmentation. Plant J. 78 , 294–304 (2014). Article CAS PubMed Google Scholar WebMay 18, 2024 · Naringenin is an essential precursor for all flavonoids, and effectively promoting naringenin production is crucial in metabolic engineering. The interaction between plant metabolic enzymes ensures metabolic flux. The effect can effectively improve the natural product synthesis of engineering microbial systems. In this study, chalcone …
A chalcone isomerase-like protein enhances flavonoid production …
The cDNAs encoding flavonoid enzymes and CHILs analyzed in this study are summarized in Supplementary Table 4. The cDNAs encoding flavonoid enzymes of snapdragon, torenia, and soybean [i.e., CHS, chalcone reductase (CHR, soybean), CHI, 2-hydroxyisoflavanone dehydratase (HID, soybean), FNSII … See more The plasmids used in this study were obtained from the following sources: pGEM-T Easy vector (Promega, Madison, WI, USA); pGBKT7 and pGADT7 (each used for the GAL4-based yeast two-hybrid assays) and pET15b … See more The THC and p-coumaroyl-CoA were purchased from TransMIT (Gießen, Germany). Malonyl-CoA, naringenin, trans-p-coumaric acid, resveratrol, ampicillin, imidazole, … See more The Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA) was used to complete GAL4-based yeast two-hybrid assays as previously described8,9,10. Briefly, the open reading frames encoding … See more The interactions between CHILs and cytochromes P450 from snapdragon and soybean (AmFNSII, GmIFS-1, and GmIFS-2) were analyzed in the split-ubiquitin system with … See more WebFeb 2, 2024 · Chalcone isomerase (CHI) is an important enzyme for flavonoid biosynthesis that catalyzes the intramolecular cyclization of chalcones into (S)-flavanones. CHIs have been classified into two types based on their substrate specificity. Type I CHIs use naringenin chalcone as a substrate and are found in most of plants besides legumes, … cully 62028j
Noncatalytic chalcone isomerase-fold proteins in - PNAS
WebFeb 10, 2024 · For example, in diverse plant species, catalytically inactive type-IV chalcone isomerase-like protein serves as an enhancer of flavonoid production via its involvement in flavonoid metabolons. In soybean roots, a specific isozyme of chalcone reductase (CHR) interacts with 2-hydroxyisoflavanone synthase, to which chalcone synthase (CHS) can … WebMar 25, 2024 · Gene ID: 4334588, updated on 25-Mar-2024. Summary Other designations. chalcone--flavanone isomerase, chalcone--flavonone isomerase, Chalcone--flavanone isomerase, LOC_Os03g60509, chalcone isomerase. GeneRIFs: Gene References Into Functions. findings demonstrated that the OsCHI gene was indispensable for flux of the … WebIn order to investigate the role of chalcone isomerase in anthocyanins biosynthesis in sweetpotato, we cloned and characterized the chalcone isomerase gene from purple-fleshed sweetpotato (Ipomoea batatas (L.) Lam) cultivar Yuzi 263, which was designated as IbCHI (Genbank accession number: JN083840). The full-length cDNA of IbCHI was 890 … east hanover pba